Additional Selective and Differential Cell Culture Media
NEOGEN Glucose OF Medium, 500 g
Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-of-medium
NEOGEN Glucose OF Medium, 5 kg
Glucose OF Medium is used for the confirmation of Enterobacteriaceae in foods according to ISO 21528-1&2:2017, and is not intended for use in the diagnosis of disease or other conditions in humans.Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator.Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae.For more information visit https://www.neogen.com/solutions/Microbiology/glucose-of-medium
NEOGEN DNase Agar, 500 g
DNase Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity and is not intended for use in the diagnosis of disease or other conditions in humans.In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that degrade DNA produce a clear zone around an inoculum streak.For more information visit https://www.neogen.com/solutions/Microbiology/dnase-agar
NEOGEN Buffered Listeria Enrichment Broth Base, 10 kg
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-listeria-enrichment-broth-base
NEOGEN Sorbitol MacConkey Agar, 5 kg
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Universal Beer Agar, 500 g
Universal Beer Agar is used for the cultivation of bacteria and yeasts encountered in the brewing industry in a laboratory setting. Universal Beer Agar is not intended for use in the diagnosis of disease or other conditions in humans. Universal Beer Agar was developed by Kozulis and Page and used as a basal medium supplemented with beer. The developers compared this formula with other media commonly used in breweries for detecting microbial contamination. The characteristics of Universal Beer Agar are closer to natural environmental conditions found in a typical brewery. Universal Beer Agar supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than traditional brewer’s media. Supplementing this medium with beer has produced a selective environment for organisms that are adapted to existent conditions in the brewery.For more information visit https://www.neogen.com/solutions/Microbiology/universal-beer-agar
NEOGEN Sorbitol MacConkey Agar, 500 g
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Universal Beer Agar, 10 kg
Universal Beer Agar is used for the cultivation of bacteria and yeasts encountered in the brewing industry in a laboratory setting. Universal Beer Agar is not intended for use in the diagnosis of disease or other conditions in humans. Universal Beer Agar was developed by Kozulis and Page and used as a basal medium supplemented with beer. The developers compared this formula with other media commonly used in breweries for detecting microbial contamination. The characteristics of Universal Beer Agar are closer to natural environmental conditions found in a typical brewery. Universal Beer Agar supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than traditional brewer’s media. Supplementing this medium with beer has produced a selective environment for organisms that are adapted to existent conditions in the brewery.For more information visit https://www.neogen.com/solutions/Microbiology/universal-beer-agar
NEOGEN Alkaline Saline Peptone Water (ASPW) (ISO), 500 g
Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.For more information visit https://www.neogen.com/solutions/Microbiology/alkaline-saline-peptone-water-iso
NEOGEN Yersinia Slective Agar (Schiemann's CIN Agar), 500 g
Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans. This medium is based on the work of Schiemann. It is used for the isolation and enumeration of Yersinia spp. from clinical samples and from food. The selective components are sodium desoxycholate, crystal violet, cefsulodin, irgasan and novobiocin. Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance. The ratio of transparent border to red centre varies with serotype and environmental strains may appear rough with an irregular edge. Most other enteric bacteria, if they grow, produce a larger colony with a diffuse pinkish center and opaque outer zone.For more information visit https://www.neogen.com/solutions/Microbiology/yersinia-selective-agar
NEOGEN Slanetz and Bartley Agar, 500 g
Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).For more information visit https://www.neogen.com/solutions/Microbiology/slanetz-bartley-medium
NEOGEN MacConkey Broth Purple, 500 g
MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans.The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.For more information visit https://www.neogen.com/solutions/Microbiology/macconkey-broth-purple
NEOGEN Kanamycin Aesculin Azide Agar, 500 g
Kanamycin Aesculin Azide Agar is a selective isolation and enumeration medium for enterococci (Lancefield group D streptococci) in food, and is not intended for use in the diagnosis of disease or other conditions in humans.Sodium azide and kanamycin provide the selective inhibition required whilst aesculin and iron salts form an indicator system for the presumptive identification of enterococci. Incubation at 42°C will increase the medium’s selectivity.Formula:Tryptone - 20.0 g/LYeast Extract - 5.0 g/LSodium Chloride - 5.0 g/LSodium Citrate - 1.0 g/LAesculin - 1.0 g/LFerric Ammonium Citrate - 0.5 g/LSodium Azide - 0.15 g/LKanamycin Sulphate - 0.02 g/LAgar- 10.0 g/LFor more information visit https://www.neogen.com/solutions/Microbiology/kanamycin-aesculin-azide-agar
NEOGEN LESS Plus Medium Irradiated, 500 g
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicates the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes. Implicated vehicles of transmission include meat, eggs, chicken, vegetables, and dairy products. Listeria spp. are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river water. Certain strains of Listeria spp. are able to survive the manufacturing and ripening processes in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/less-plus-medium
NEOGEN Sodium Chloride (Bacteriological), 10 kg
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological